Melting Point Determination in Practice

In almost every chemistry, pharmacy or materials laboratory, there is a quiet moment when everything stops. The sample in the tiny capillary begins to soften, collapse, and finally turn into a clear liquid. This short temperature interval — the melting point — remains one of the most reliable tools for understanding organic substances, medicines and fine chemicals.

For pure crystalline substances, the melting point behaves almost like a fingerprint. It confirms identity, highlights impurities and reveals polymorphs or degradation. Modern pharmacopoeias describe it as a melting range — from the first visible change to the moment when the last crystal disappears. Because of this, a good melting point result is more than a single number. It is a controlled experiment with well-defined conditions, stable heating and proper documentation.

From capillary tubes to digital video melting curves

Historically, melting points were measured with a thermometer and an oil bath. The operator watched the sample closely and noted the first and last signs of melting. Today, modern instruments automate the process while remaining aligned with standards such as European Pharmacopoeia 2.2.14 and USP <741>. At COLO.Science, we focus on three complementary platforms:

• CMPA1 – Visual Melting Point with Microscope Ideal for studying melting behaviour, training new users or resolving ambiguous automated results.
• CMPA3 – Fully Automatic Micro-Processor Melting Point Designed for routine QC, reproducible results and stable heating control.
• CMPA7 – Advanced Image-Analysis Video Melting Point A high-end system with AUTO-ROI® detection, HD camera, video recording, curves and full data integrity tools.

What do we really measure at the melting point?

Most substances do not melt at a single temperature. Instead, they move through a short transition that includes softening, meniscus formation and coexistence of solid and liquid phases. Pharmacopoeias therefore define onset, melting range and clear point, and they describe strict ramp rates, sample height and capillary dimensions. Temperature stability and precise heating control are essential for reliable results.

Standards, reference materials and calibration

Pharmacopoeial chapters describe how to verify accuracy using certified reference substances. They define heating rate tolerances, capillary dimensions and sample height. All COLO instruments are designed for these requirements. Ramp rates can follow pharmacopeial values, and results can be exported or printed for complete QC documentation.

Melting point at COLO.Science

On COLO.Science we explore melting points not only as a number but as a view into solid-state behaviour. Whether you are qualifying a new API, comparing suppliers, teaching students or preparing for an inspection, a well-designed melting point method increases confidence in your results. For support or instrument suggestions, you can contact our team at sales@colo.si.